A pilot analysis of small nucleolar rna editome of oxidative stressed retinal epithelial cells revealed a possible role for dysregulated neurotransmission in inherited retinal diseases

A pilot analysis of small nucleolar rna editome of oxidative stressed retinal epithelial cells revealed a possible role for dysregulated neurotransmission in inherited retinal diseases

Luigi Donato, Concetta Scimone, Simona Alibrandi, Domenico Mordà, Alessandra Costa, Fabiana Nicita, Rosalia D’angelo, Antonina Sidoti


The growing knowledge on non-coding RNAs (ncRNAs) suggests that a significant number of transcriptional and posttranscriptional regulative mechanisms in eukaryotes could represent the key to improve our knowledge on many diseases etiopathogenesis, like inherited retinal diseases (IRDs). Among ncRNAs, small nucleolar RNAs (snoRNAs) is still an unexplored world. We investigated the posttranscriptional RNA editing landscape of human retinal pigment epithelium cells (RPE) exposed to the oxidant agent N-retinylidene-N-retinyl ethanolamine (A2E) for 3 h and 6 h, focusing on snoRNA host and target genes. Using a transcriptomic approach, refined with a specific multialgorithm pipeline, 9 snoRNA editing sites within related host genes were identified among all samples. Such genes resulted involved in several biochemical pathways linked to retinal neurotransmission. SnoRNA epitranscriptome analysis of oxidative stress induced RPE cells suggested that such ncRNAs could play a relevant role in IRD etiopathogenesis, regulating pathways directly or indirectly related to the considered disease.

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